Chromatin structure of simian virus 40-pBR322 recombinant plasmids in COS-1 cells.
نویسندگان
چکیده
منابع مشابه
Archetype JC virus efficiently replicates in COS-7 cells, simian cells constitutively expressing simian virus 40 T antigen.
JC polyomavirus (JCV), the causative agent of progressive multifocal leukoencephalopathy (PML), is ubiquitous in humans, infecting children asymptomatically and then persisting in the kidney. Renal JCV is not latent but replicates to excrete progeny in the urine. The renal-urinary JCV DNAs carry the archetype regulatory region that generates various rearranged regulatory regions occurring in JC...
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We have studied the binding of the tumor antigen (T-antigen) of simian virus 40 to simian virus 40 chromatin (minichromosomes). The minichromosomes isolated from infected cells by a modification of standard techniques were relatively free of contaminating RNA and cellular DNA and had a ratio (by weight) of protein to DNA of approximately 1; their DNA was 50 to 60% digestible to an acid-soluble ...
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Three simian virus (SV40)-phi X174 recombinant genomes were isolated from single BSC-1 monkey cells cotransfected with SV40 and phi X174 RF1 DNAs. The individual cell progenies were amplified, cloned, and mapped by a combination of restriction endonuclease and heteroduplex analyses. In each case, the 600 to 1,000 base pairs of phi X174 DNA (derived from different regions of the phi X174 genome)...
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The complete nucleotide sequence has been determined for three newly cloned evolutionary variants from two different independently generated evolutionary series ( 1 100 and 2100 series) of simian virus 4 0 (SV40). These naturally arising variants, designated ev-1110, ev-2102, and eu-2114, were isolated after five high multiplicity serial passages. The structure of the variants consists of a mon...
متن کاملRecombination in Simian Virus 40-infected Cells
The complete nucleotide sequence has been determined for three newly cloned evolutionary variants from two different independently generated evolutionary series ( 1 100 and 2100 series) of simian virus 4 0 (SV40). These naturally arising variants, designated ev-1110, ev-2102, and eu-2114, were isolated after five high multiplicity serial passages. The structure of the variants consists of a mon...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1983
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.3.12.2203